图1  蛋白质GO功能注释

经典案例

橡胶粒子蛋白质组全谱分析  Plant Mol Biol. 2013.

In-depth proteome analysis of the rubber particle of Heveabrasiliensis (para rubber tree). Plant Mol Biol. 2013, doi: 10.1007/s11103-013-0047-y.

本文利用两种不同的蛋白质全谱分析技术（GeLC-MS/MS和MudPIT），对巴西橡胶（H. brasiliensis）粒子的蛋白质在进行了全谱分析，共鉴定出186个非冗余蛋白，其中REF和SRPP等蛋白及其同系蛋白与橡胶合成相关，其表达趋势与其转录组数据一致。功能分析显示，许多蛋白特别是在橡胶树乳汁管中表达的蛋白，其功能未知或不清晰，与转录组研究结果一致。

图1  鉴定的橡胶粒子蛋白venn图

图2  Cyclophilin A 的LC-MRM-MS分析结果

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